First squaraine rotaxane with Alexa 555 and Cy3 emission wavelengths but much superior properties!

We invite you to look at SeTau-500-NHS (Φ = 0.43,  ε = 59,600 M^-1cm^-1 and Φ∙ε = 25,628 M^-1cm^-1), our latest addition to our rotaxane dyes with an absorption at 500 nm and an emission at 560 nm, similar to Cy3 or Alexa-555 but with a quantum yield several times higher than Cy3 (Φ = 0.1,  ε = 162,000 M^-1cm^-1 and Φ∙ε = 16,200 M^-1cm^-1) or Alexa 555 (Φ = 0.1,  ε = 152,000 M^-1cm^-1 and Φ∙ε = 15,200 M^-1cm^-1). It also exhibits a lower quenching tendency when conjugated to biomolecules and features an exceptionally large Stokes' shift of 60 nm, which can enhance the measurement sensitivity by enabling the use of broader filter sets! Exc./Em. max of IgG-conjugates: 500/663 nm. Try it yourself now!

 

Total brightness (QY × ε × D/P) vs. dye-to-protein ratio (D/P)
of SeTau-500 — IgG conjugates in PBS (pH 7.4) as compared to Alexa 488 — IgG and Cy3 — IgG conjugates

 

The dye is available in 0.25 mg, 0.5 mg and 1 mg product sizes!

			Excitation Light Sources												Characteristics
Product Number (Specs Sheet)	Product Name (Product Info)	Target Group	380	405	436	488	532	594	635	650	680	700	750	780	Medium	λ abs [nm]	ε [M –1 cm–1]	λ em [nm]	QY [%]	FLT [ns]	Buy
K8-3335 K8-3335	Seta-555-NHS Seta-555-NHS	NH2					•								PB 7.4	555	155000	570	7	0.3
K9-4149 K9-4149	SeTau-647-NHS SeTau-647-NHS	NH2							•	•					PB 7.4	649	200000	695	61	3.2
K9-4169 K9-4169	SeTau-670-NHS SeTau-670-NHS	NH2							•	•	•				PB 7.4	673	275000	694	36	1.6
K9-4179 K9-4179	SeTau-680-NHS SeTau-680-NHS	NH2							•	•	•				PB 7.4	683	295000	705	58	2.9
K9-3152 K9-3152	SeTau-488-NHS SeTau-488-NHS	NH2				•									PBS 7.4	486	59000	532	27	1.2
K9-3153 K9-3153 new	SeTau-488-Maleimide SeTau-488-Maleimide	SH				•									PBS 7.4	484	59000	531	14
K9-3162 K9-3162 new	SeTau-500-NHS SeTau-500-NHS	NH2				•									PB 7.4	502	59600	567	43	2.8